abstract：OBJECTIVES:Blood sampling/handling alters matrix metalloproteinases (MMP) and tissue inhibitors of metalloproteinases (TIMP) expression. The aim of this study is to evaluate the effects of high molecular weight heparin on MMP and TIMP expression in blood. DESIGN AND METHODS:We analyzed by gelatin zymography and ELISA ...

journal_title：Clinical biochemistry

authors： Mannello F,Jung K,Tonti GA,Canestrari F

更新日期：2008-12-01 00:00:00

abstract：OBJECTIVE:The aim of this study was to find a pre-interventional marker with the capacity to predict in-stent restenosis (ISR). Considering the anti-atherosclerotic role of adiponectin (APO), an adipocytokine with anti-inflammatory, anti-proliferative, anti-oxidative and anti-thrombotic properties, low plasma levels of...

journal_title：Clinical biochemistry

authors： Moldoveanu E,Mut-Vitcu B,Tanaseanu GR,Marta DS,Manea G,Kosaka T,Vidulescu C,Tanaseanu C

更新日期：2008-12-01 00:00:00

abstract：OBJECTIVES:An increased level of serum vascular adhesion protein-1 (VAP-1) has been found in patients with diabetes mellitus and vascular disorders. This study examined whether serum VAP-1 levels are associated with chronic kidney disease (CKD). DESIGN AND METHODS:We included 262 subjects aged 30 and above with fastin...

journal_title：Clinical biochemistry

authors： Lin MS,Li HY,Wei JN,Lin CH,Smith DJ,Vainio J,Shih SR,Chen YH,Lin LC,Kao HL,Chuang LM,Chen MF

更新日期：2008-11-01 00:00:00

abstract：OBJECTIVES:This study investigates how some of human physiological parameters, in particular sex and daytime, can contribute to the normal variation in blood gene expression. DESIGN AND METHODS:The expressions of two genes: SOD1 (Cu/Zn) and SOD2 (Mn) in white blood cells of 19 healthy individuals (10 men and 9 women) ...

journal_title：Clinical biochemistry

authors： Khymenets O,Covas MI,Farré M,Langohr K,Fitó M,de la Torre R

更新日期：2008-11-01 00:00:00

abstract：OBJECTIVES:To evaluate the evidence concerning gFOBT kits and to develop gFOBT standards to be implemented by the Ontario Colorectal Cancer (CRC) Screening Program (the "Program"). DESIGN AND METHODS:We evaluated 3 aspects of gFOBT kits: 1) performance factors; 2) usability factors; and 3) laboratory factors. We obtai...

journal_title：Clinical biochemistry

authors： Rabeneck L,Zwaal C,Goodman JH,Mai V,Zamkanei M

更新日期：2008-11-01 00:00:00

abstract：OBJECTIVES:To compare a fully automated immunoassay and a fully automated HPLC method for the quantification of HbA1c. DESIGN AND METHODS:For an inter-method comparison, 200 consecutive clinical samples were analyzed. Imprecision was studied with pooled whole blood. RESULTS:Very close agreement of results (identical ...

journal_title：Clinical biochemistry

authors： Vogeser M,Parhofer KG

更新日期：2008-11-01 00:00:00

abstract：OBJECTIVE:Determine the relation of xanthine oxidase (XO) activity and the outcome of septic patients and its relation to oxidative damage and clinical parameters of sepsis severity. DESIGN AND METHODS:Patients admitted over a 6-month period were enrolled. Patients were assigned to groups according to the diagnosis of...

journal_title：Clinical biochemistry

authors： Luchtemberg MN,Petronilho F,Constantino L,Gelain DP,Andrades M,Ritter C,Moreira JC,Streck EL,Dal-Pizzol F

更新日期：2008-10-01 00:00:00

abstract：OBJECTIVES:To compare the accuracy of procalcitonin (PCT) in early-onset neonatal sepsis (EOS) using standard cut-off values and a multilevel probabilistic approach. DESIGN AND METHODS:A retrospective study of PCT was performed in 149 newborns at risk of EOS, including preterm or prolonged rupture of membranes, chorio...

journal_title：Clinical biochemistry

authors： Santuz P,Soffiati M,Dorizzi RM,Benedetti M,Zaglia F,Biban P

更新日期：2008-10-01 00:00:00

abstract：BACKGROUND:L-Dopa decarboxylase (DDC) is a pyridoxal 5'-phosphate-dependent enzyme that was found to be involved in many malignancies. The aim of this study was to investigate the mRNA expression levels of DDC in prostate tissues and to evaluate its clinical utility in prostate cancer (CaP). METHODS:Total RNA was isol...

journal_title：Clinical biochemistry

authors： Avgeris M,Koutalellis G,Fragoulis EG,Scorilas A

更新日期：2008-10-01 00:00:00

abstract：BACKGROUND:A determination of serum Transthyretin (TTR, Prealbumin) level is an objective method of assessing protein catabolic loss of severely ill patients and numerous studies have shown that TTR levels correlate with patient outcomes of non-critically ill patients. We evaluated whether TTR level correlates with the...

journal_title：Clinical biochemistry

authors： Devakonda A,George L,Raoof S,Esan A,Saleh A,Bernstein LH

更新日期：2008-10-01 00:00:00

abstract：OBJECTIVES:To compare the circulating levels of matrix metalloproteinase (MMP)-2, MMP-9, tissue inhibitors of metalloproteinase (TIMP)-1, TIMP-2, and the MMP-9/TIMP-1 and MMP-2/TIMP-2 ratios in preeclampsia and gestational hypertension with those found in normotensive pregnancies. DESIGN AND METHODS:We studied 83 preg...

journal_title：Clinical biochemistry

authors： Palei AC,Sandrim VC,Cavalli RC,Tanus-Santos JE

更新日期：2008-07-01 00:00:00

abstract：UNLABELLED:Free radical production and high catecholamine levels are implicated with the modulation of acetylcholinesterase (AChE) activity. OBJECTIVE:To investigate the effect of the mode of delivery on maternal-neonatal erythrocyte membrane AChE activity. SUBJECTS AND METHODS:Some women with normal pregnancy (N = 3...

journal_title：Clinical biochemistry

authors： Vlachos DG,Schulpis KH,Parthimos T,Mesogitis S,Vlachos GD,Partsinevelos GA,Antsaklis A,Tsakiris S

更新日期：2008-07-01 00:00:00

abstract：OBJECTIVE:To quantitatively summarize the association between tumour necrosis factor alpha (TNF-alpha) gene polymorphisms and Graves' disease. DESIGN AND METHODS:Relevant studies were identified from the following electronic databases: Cochrane Library, MEDLINE, EMBASE and Chinese Bio-medicine Database. A meta-analysi...

journal_title：Clinical biochemistry

authors： Li N,Zhou Z,Liu X,Liu Y,Zhang J,Du L,Wei M,Chen X

更新日期：2008-07-01 00:00:00

abstract：BACKGROUND:Exercise is a prone condition to enhanced oxidative stress and damage and the specific activity pattern of a soccer match may favour additional pro-oxidant redox alterations. To date, no studies have reported the impact of a soccer match on oxidative stress and muscle damage markers. AIM:To analyse the effe...

journal_title：Clinical biochemistry

authors： Ascensão A,Rebelo A,Oliveira E,Marques F,Pereira L,Magalhães J

更新日期：2008-07-01 00:00:00

abstract：OBJECTIVES:Despite the well-defined genetics of FMF, limited information is available regarding the regulation of inflammation by cytokines. DESIGN AND METHODS:The levels of systemic cytokines and other markers of inflammation in FMF patients and control were measured by ELISA and Cytometric Bead Array (CBA). RESULTS...

journal_title：Clinical biochemistry

authors： Manukyan GP,Ghazaryan KA,Ktsoyan ZhA,Tatyan MV,Khachatryan ZA,Hakobyan GS,Mkrtchyan VA,Kelly D,Coutts A,Aminov RI

更新日期：2008-07-01 00:00:00

abstract：:During the past decade, tandem mass spectrometry hyphenated to liquid chromatography separation systems (HPLC-MS/MS) has developed to an important technology in clinical chemistry - not only for research purposes but also for routine use. At present, most important application fields are target analyses in therapeutic...

journal_title：Clinical biochemistry

authors： Vogeser M,Seger C

更新日期：2008-06-01 00:00:00

abstract：OBJECTIVES:The steroids estradiol (E2), estrone (E1), and estriol (E3) are the major estrogens. E1/E2 and their metabolite 16-hydroxyestrone (16-OHE1, known to be carcinogenic) could be involved in the development of many cancers including human breast cancer. The aim of the current study was to develop a rapid and sim...

journal_title：Clinical biochemistry

authors： Guo T,Gu J,Soldin OP,Singh RJ,Soldin SJ

更新日期：2008-06-01 00:00:00

abstract：OBJECTIVE:Salivary cortisol in the assessment of glucocorticoid related disorders. DESIGN-METHODS: Serum and salivary cortisol were measured in 189 patients (22 Cushing's syndrome, 67 pseudo-Cushing, 11 Addison's disease, 89 controls) at 8:00 and 24:00 h. RESULTS:Serum and salivary cortisol correlated in the whole stu...

journal_title：Clinical biochemistry

authors： Restituto P,Galofré JC,Gil MJ,Mugueta C,Santos S,Monreal JI,Varo N

更新日期：2008-06-01 00:00:00

abstract：OBJECTIVES:Angiotensin converting enzyme (ACE), G-Protein couple receptor (G-Prot), endothelial nitric oxide synthase (ecNOS), Leptin -2548G/A and uncoupling protein (UCP2) are potent regulators of intra renal hemodynamics and may be the causative factors contributing to the deterioration of renal functions. In recent ...

journal_title：Clinical biochemistry

authors： Tripathi G,Sharma RK,Baburaj VP,Sankhwar SN,Jafar T,Agrawal S

更新日期：2008-05-01 00:00:00

abstract：OBJECTIVES:To test for specific mutations in the alanine:glyoxylate aminotransferase (AGT) gene, in order to diagnose primary hyperoxaluria type 1 (PH1). DESIGN AND METHODS:Samples of liver and/or DNA from 81 patients were submitted to our laboratory for diagnostic testing for PH1. Using a panel of selected mutations,...

journal_title：Clinical biochemistry

authors： Coulter-Mackie MB,Lian Q,Applegarth DA,Toone J,Waters PJ,Vallance H

更新日期：2008-05-01 00:00:00

abstract：OBJECTIVES:To examine whether the proportions of blood leukocyte subsets are altered in COPD, and to assess the disturbances in signalling pathways in the leukocytes of COPD patients, with respect to smoking history. DESIGN AND METHODS:The study was performed at the Faculty of Pharmacy and Biochemistry and University ...

journal_title：Clinical biochemistry

authors： Rumora L,Milevoj L,Popović-Grle S,Barisić K,Cepelak I,Grubisić TZ

更新日期：2008-04-01 00:00:00

abstract：OBJECTIVES:Pseudoxanthoma elasticum (PXE) is a hereditary disorder predominantly affecting the skin, retina and vascular system. The aim of this study was to measure cell adhesion molecules in PXE patients. DESIGN AND METHODS:Soluble P-, E- and L-selectins were measured in 61 non-consanguineous PXE patients. The distr...

journal_title：Clinical biochemistry

authors： Götting C,Adam A,Szliska C,Kleesiek K

更新日期：2008-04-01 00:00:00

abstract：OBJECTIVES:The fibroblast growth factor receptor 3 gene (FGFR3) plays a critical role in cartilage growth-plate differentiation and bony development. It has been shown that 97% of patients with achondroplasia have a G to A transition mutation at position 1138 (c.1138 G>A) of codon 380 of the FGFR3 gene. DESIGN AND MET...

journal_title：Clinical biochemistry

authors： Hung CC,Lee CN,Chang CH,Jong YJ,Chen CP,Hsieh WS,Su YN,Lin WL

更新日期：2008-02-01 00:00:00

abstract：BACKGROUND:Polycyclic aromatic hydrocarbons (PAHs), some ubiquitous environmental contaminants are capable to cause oxidative stress, during its metabolism. It is believed that many diseases that have a common origin in oxidative stress begin in childhood. Considering oxidative stress evolved during PAHs metabolism as ...

journal_title：Clinical biochemistry

authors： Singh VK,Patel DK,Jyoti,Ram S,Mathur N,Siddiqui MK

更新日期：2008-02-01 00:00:00

abstract：OBJECTIVE:Heat-mediated ELISA (HELISA) technique has been reported to shorten ELISA timings and has major clinical implications in diagnostic field [Bora U, Kannan K, Nahar P. Heat mediated enzyme linked immunosorbent assay. J Immunol Methods 2004; 293: 43-50]. Objective of this study is to find out whether anti-MBL au...

journal_title：Clinical biochemistry

authors： Sharma P,Gupta B,Farhat Basir S,Rani Das H,Nahar P

更新日期：2008-01-01 00:00:00

abstract：OBJECTIVES:Superoxide dismutase (SOD) is a key antioxidant enzyme, responsible for scavenging of superoxide anion - a precursor of all reactive oxygen species (ROS). ROS are implicated in many pathologies, particularly in tumor disease. The aim of our work was to evaluate SOD isoforms' activity and protein level change...

journal_title：Clinical biochemistry

authors： Skrzycki M,Scibior D,Podsiad M,Czeczot H

更新日期：2008-01-01 00:00:00

abstract：OBJECTIVES:The clinical analysis of hemoglobin by ion exchange chromatography can result in ambiguities in identification of the nature of the globin chain present in patient samples. LC/ESI-MS provides rapid and precise determination of globin chain masses. DESIGN AND METHODS:Hemolysate of hemoglobin Q-India and hemo...

journal_title：Clinical biochemistry

authors： Mandal AK,Bisht S,Bhat VS,Krishnaswamy PR,Balaram P

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Patients suffering from hemorrhagic fever with renal syndrome (HFRS) often showed strikingly reduced high-density lipoprotein (HDL)-cholesterol levels during the oliguric phase, indicating severe alterations in lipoprotein metabolism. OBJECTIVE:To compare changes in the functions and composition of HDL, lip...

journal_title：Clinical biochemistry

authors： Cho KH,Park SH,Park JE,Kim YO,Choi I,Kim JJ,Kim JR

更新日期：2008-01-01 00:00:00

abstract：OBJECTIVES:Current extraction methods for cobalamins from serum influence the molecular characteristics of the vitamin. Therefore, an extraction procedure that leaves the cobalamins unchanged is needed. DESIGN AND METHODS:Monoclonal antibodies towards transcobalamin (TC) and haptocorrin (HC) (in house) linked to magne...

journal_title：Clinical biochemistry

authors： Hardlei TF,Morkbak AL,Nexo E

更新日期：2007-12-01 00:00:00

abstract：OBJECTIVE:In this study, we determined the assay performance criteria necessary to produce acceptable results for >or=98% of neonate bilirubin samples collected by capillary heel-stick. STUDY DESIGN AND METHODS:We determined serum free hemoglobin levels in 151 heel-stick serum samples to determine the hemolysis level....

journal_title：Clinical biochemistry

authors： Algeciras-Schimnich A,Cook WJ,Milz TC,Saenger AK,Karon BS

更新日期：2007-11-01 00:00:00

abstract：OBJECTIVES:We established population-based reference limits for plasma total homocysteine (tHcy) according to creatinine. DESIGN AND METHODS:In 7042 middle-aged and elderly subjects from the Hordaland Homocysteine Study, we used statistical modeling to establish nomograms for tHcy according to creatinine in the whole ...

journal_title：Clinical biochemistry

authors： Elshorbagy AK,Oulhaj A,Konstantinova S,Nurk E,Ueland PM,Tell GS,Nygård O,Vollset SE,Refsum H

更新日期：2007-11-01 00:00:00

abstract：OBJECTIVE:The aim of this study was to determine the adult reference values for cystatin C (CysC) and to evaluate their consistence with those reported in the literature. DESIGN AND METHODS:CysC was analyzed in a consecutive series of subjects (100 males and 100 females) by a nephelometric immunoassay. Medline was sea...

journal_title：Clinical biochemistry

authors： Croda-Todd MT,Soto-Montano XJ,Hernández-Cancino PA,Juárez-Aguilar E

更新日期：2007-09-01 00:00:00

abstract：OBJECTIVES:Plasma retinol-binding protein (RBP) has been linked to insulin resistance and cardiovascular risk, yet little is know of its natural variation in plasma. We examined this in normal subjects and compared plasma levels and variability in lean subjects and subjects with the metabolic syndrome. METHODS:We esta...

journal_title：Clinical biochemistry

authors： Lewis JG,Shand BI,Frampton CM,Elder PA

更新日期：2007-07-01 00:00:00

abstract：OBJECTIVES:Does abnormal betaine excretion persist? DESIGN AND METHODS:Patients (10) with abnormal excretion in 1998 were recalled in 2005. Subsequent urine samples were collected on 4 days from persistently abnormal subjects. RESULTS:Half the 1998 abnormal patients were abnormal in 2005. Only 1/20 controls was abnor...

journal_title：Clinical biochemistry

authors： Lever M,Atkinson W,George PM,Chambers ST

更新日期：2007-07-01 00:00:00

abstract：BACKGROUND:Cystinosis is a rare autosomal recessive disorder characterized by an accumulation of intralysosomal cystine due to a defect in cystine transport across the lysosomal membrane. This disorder can be treated specifically using high doses of cysteamine. Accurate measurement of intracellular cystine content is n...

journal_title：Clinical biochemistry

authors： Chabli A,Aupetit J,Raehm M,Ricquier D,Chadefaux-Vekemans B

更新日期：2007-06-01 00:00:00

abstract：OBJECTIVE:To evaluate the clinical use of Homocysteine-Primavette, a new blood collection medium for total homocysteine (tHcy) assay. METHODS:The agreement between baseline tHcy and tHcy in stabilized samples (40 h) was assessed for FPIA, HPLC, GC-MS, LC-MS, and ICL. RESULTS:tHcy concentrations in whole blood were st...

journal_title：Clinical biochemistry

authors： Bissé E,Epting T,Kögel G,Obeid R,Gempel K,Huzly D,Fuhrmann K,Rolinski B,Herrmann W,Wieland H

更新日期：2007-06-01 00:00:00

abstract：OBJECTIVES:Glycosylation analysis provides many opportunities for diagnostics, but its complexity hampers its routine application. Aiming to alleviate this problem, we developed a simple assay that can measure sialylation of transferrin directly from serum. DESIGN AND METHODS:Transferrin samples with different levels ...

journal_title：Clinical biochemistry

authors： Gornik O,Lauc G

更新日期：2007-06-01 00:00:00

abstract：OBJECTIVE:Plasma ammonia measurement is greatly influenced by pre-analytical conditions, which may lead to false positives. We wanted to evaluate the prevalence and clinical impact of plasma ammonia false positives in a pediatric population. DESIGN AND METHODS:Over a 28-month period, charts of patients with elevated a...

journal_title：Clinical biochemistry

authors： Maranda B,Cousineau J,Allard P,Lambert M

更新日期：2007-05-01 00:00:00

abstract：OBJECTIVES:To establish whether normal human subjects excrete glycine betaine at a constant rate. DESIGN AND METHODS:Urine was collected from ten normal healthy male subjects for 14 days, during which fluid intake was systematically varied from <800 mL to >3 L per day. Glycine betaine, sorbitol and creatinine excretio...

journal_title：Clinical biochemistry

authors： Lever M,Atkinson W,Sizeland PC,Chambers ST,George PM

更新日期：2007-04-01 00:00:00

abstract：OBJECTIVES:The purpose of this study is to assess the usefulness of the concentration of cystatin C (Cys-C) in serum for predicting the clearance of vancomycin (CLvcm) compared with the serum concentration of creatinine (SCr) in the elderly. METHODS:Thirty-nine serum samples were obtained from 24 elderly patients (65 ...

journal_title：Clinical biochemistry

authors： Okamoto G,Sakamoto T,Kimura M,Ukishima Y,Sonoda A,Mori N,Kato Y,Maeda T,Kagawa Y

更新日期：2007-04-01 00:00:00